Goals from promising youngster Carlton Cox and John Grant sealed a 2-0 Shots victory on the opening day, and even with injuries hitting Gary Waddock's squad in recent weeks, the ethos has remained the same and Gary Waddock has confirmed that yet again, the strongest team possible will be chosen for the match.
"We had a good win on Saturday but as usual, we'll have to sit down on Monday morning and work out who's available to us" he told the clubs official website.
"It's no secret that we're working with a small squad but there are some good youngsters coming through the ranks and these games are vital for their progression.
"They're also great for those players who are looking to get back into the first team and Brentford will be treating the match in much the same way as us."
Brentford from: Nikki Bull, Fraser Franks, Lewis Ferrell, Marcus Bean, Karleigh
Osbourne, Leon Legg, Cleveland Taylor, James McCluskey, Marvin Williams, Steve Kabba, Simon Moore, Bilal Butt, Geoff Banks-Smith, Charlie Allen and Ross Montague.
Shots from: Clark Masters, Dan Smith, Josh Casey, Ben Hutchings, Lewis Chalmers, Carlton Cox, Josh Pearson, Bobby Hopkinson, Reece Connolly, Adam Mekki, Max Worsfold, John Grant, Calum Nimmo, Luke Hutchings and James Pilgrim.
Entry to Griffin Park is £3 for adults and £1 for concessions.
Tuesday's Other Combination (Central) Fixtures
QPR vs Gillingham, kick-off 2pm
Millwall vs Crystal Palace, kick-off 7pm
Next Up For The Reserves
The Shots Reserves are next in action at the EBB Stadium on Wednesday 7th October 2009, when they face Wessex League side Romsey Town in the Second Round of the Hampshire Senior Cup, kick-off 7.45pm.